KMID : 0604520140400030259
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Journal of the Society of Cosmetic Scientists of Korea 2014 Volume.40 No. 3 p.259 ~ p.268
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Quantification of Melanin Density at Epidermal Basal Layer by Using Confocal Scanning Laser Microscope (CSLM)
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Kim Dong-Hyun
Lee Sung-Ho Oh Myoung-Jin Choi Go-Woon Yang Woo-Chul Park Chang-Seo
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Abstract
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Non-invasive technologies in skin research have enabled to use a live image of living skin without a biopsy or histologic processing of tissue. Confocal scanning laser microscope (CSLM) operated at a near-infrared wavelength of 830 nm allows visualization of inner structure of skin as a non-invasive manner. According to previous researches using CSLM, melanin cap and papillary ring were clearly observed in pigmented areas between stratum basale and papillary dermis. In this study, conversional analysis of CSLM digital images into numerical estimation using scanning probe image processor (SPIP) software was attempted for the first time. It is concluded that a quantification of CSLM images can pave way to expand the field of applications of CSLM.
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KEYWORD
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CSLM, melanin content, SPIP
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